Detection of Auxotroph’s Methionine Proteus Mirabilis from Different Clinical Sources

Abstract Twenty (25%) isolates of Proteus Mirabilis were isolated from a total 80 samples patients suffering otitis media and urinary tract infections. The collected various hospitals in Al-Diwaniyah city between December 2021 March 2022. was identified using cultural biochemical tests. diagnosis confirmed by polymerase chain reaction (PCR) to investigate the 16s rRNA gene, DNA sequencing register these sequences Gene Bank-NCBI for obtaining accession numbers. Auxotroph’s methionine revealed culturing on glucose-minimal salt agar. results showed that 3/20 (15%) auxotroph’s methionine. used metF metE genes . reveal (do not have gene). performed , forward reverse primers substitution mutations genes. In conclusion, prevalence infection demonstrated this study.